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MB Sample ID: SA219980

Local Sample ID:Media.1
Subject ID:SU002375
Subject Type:Bacteria
Subject Species:Several mouse commensals
Age Or Age Range:8-16 hours

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Combined analysis:

Analysis ID AN003741
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Thermo Syncronis HILIC (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode NEGATIVE
Units AUC

Chromatography:

Chromatography ID:CH002772
Methods Filename:culture_protocol_kb.docx
Instrument Name:Thermo Vanquish
Column Name:Thermo Syncronis HILIC (100 x 2.1mm,1.7um)
Column Temperature:30C
Flow Gradient:2 stage linear gradient
Flow Rate:600uL/min
Sample Injection:2uL
Solvent A:100% water; 20 mM ammonium formate, pH 3
Solvent B:100% acetonitrile; 0.1% formic acid
Analytical Time:15 minutes
Washing Buffer:10% MeOH in H2O + 0.1% formic acid
Target Sample Temperature:4
Chromatography Type:HILIC
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