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MB Sample ID: SA235025
Local Sample ID: | 322 |
Subject ID: | SU002428 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 8-32-week-old |
Weight Or Weight Range: | 25-50 |
Gender: | Male |
Animal Animal Supplier: | JAX |
Animal Housing: | 5/cage |
Animal Light Cycle: | 12h dark/12 h light |
Animal Feed: | Special diets; see group labeling |
Animal Water: | Ad libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003822 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Ion trap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | normalized ion count/mg protein |
Chromatography:
Chromatography ID: | CH002828 |
Chromatography Summary: | 30% to 43% B from 3-8 min, then from 43% to 50% B from 8-9 min, then 50-90% B from 9-18 min, then 90-99% B from 18-26 min, then held at 99% B from 26-30 min, before returning to 30% B in 6 min and held for a further 4 min |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
Column Temperature: | 40 |
Flow Rate: | 0.2 ml/min |
Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Chromatography Type: | Reversed phase |