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MB Sample ID: SA235204
| Local Sample ID: | H32 |
| Subject ID: | SU002431 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl/6 |
| Age Or Age Range: | 20 weeks (at euthanasia) |
| Gender: | Male and female |
| Animal Animal Supplier: | Jackson Labratories |
| Animal Light Cycle: | Standard light dark cycle |
| Animal Feed: | PicoLab Rodent Diet 20, 20% protein |
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Combined analysis:
| Analysis ID | AN003826 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 |
| Column | Cogent Diamond Hydride (150 × 2.1mm,4um) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 6538 QTOF |
| Ion Mode | POSITIVE |
| Units | Peak Intensity |
Chromatography:
| Chromatography ID: | CH002831 |
| Chromatography Summary: | Samples were analyzed using an Agilent 1290 UPLC coupled to an Agilent 6538 QTOF. All samples were run in normal phase using a Cogent Diamond Hydride HILIC column (150 x 2.1 mm; 100Å pore size on a 4µm particle size). Solvents: 0.1% formic acid in water (A), 0.1% formic acid in acetonitrile (B). Elution gradient: 100 to 25% solvent B over 17 minutes, 0 to 70% solvent A over 20 minutes, at 21 minutes, B increases from 25% to 100% till 25 minutes while A decreases from 70% to 0% till 25 minutes. The last 4 minutes act as a wash. Total run time = 25 minute method (per sample) |
| Instrument Name: | Agilent 1290 |
| Column Name: | Cogent Diamond Hydride (150 × 2.1mm,4um) |
| Flow Gradient: | 100 to 25% solvent B over 17 minutes, 0 to 70% solvent A over 20 minutes, at 21 minutes, B increases from 25% to 100% till 25 minutes while A decreases from 70% to 0% till 25 minutes. The last 4 minutes act as a wash. |
| Flow Rate: | 0.400 mL/min |
| Injection Temperature: | 4C |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 25 minutes |
| Chromatography Type: | HILIC |
