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MB Sample ID: SA235269

Local Sample ID:331
Subject ID:SU002432
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:8-32-week-old
Weight Or Weight Range:25-50
Gender:Male
Animal Animal Supplier:JAX
Animal Housing:5/cage
Animal Light Cycle:12h dark/12 h light
Animal Feed:Special diets; see group labeling
Animal Water:Ad libitum

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Combined analysis:

Analysis ID AN003827
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 6460C
Column Spectra 3 μm C8SR 150 × 3 mm inner diameter, Peeke Scientific
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6460 QQQ
Ion Mode POSITIVE
Units normalized ion count/mg protein

Chromatography:

Chromatography ID:CH002832
Chromatography Summary:The gradient elution program consisted of the following profile: 0 min, 82% B; 3 min, 82% B; 4 min, 90% B, 18 min, 99% B; 25 min, 99%, 27 min, 82% B, 30 min, 82% B.
Instrument Name:Agilent 6460C
Column Name:Spectra 3 μm C8SR 150 × 3 mm inner diameter, Peeke Scientific
Column Temperature:40
Flow Rate:0.5 ml/min
Solvent A:100% water; 0.2% formic acid; 2 mM ammonium formate
Solvent B:100% methanol; 0.2% formic acid; 1 mM ammonium formate
Chromatography Type:Reversed phase
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