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MB Sample ID: SA240854
| Local Sample ID: | C18-26-neg |
| Subject ID: | SU002495 |
| Subject Type: | Plant |
| Subject Species: | Brassica napus |
| Taxonomy ID: | 3708 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003920 | AN003921 | AN003922 | AN003923 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm, 2.7um) | Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm, 2.7um) | Phenomonex Kinetex C18 (50 x 2.1mm, 2.6um) | Phenomonex Kinetex C18 (50 x 2.1mm, 2.6um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | normalized relative level | normalized relative level | normalized relative level | normalized relative level |
Chromatography:
| Chromatography ID: | CH002901 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (100 x 2.1mm, 2.7um) |
| Column Temperature: | RT |
| Flow Gradient: | Mobile phase 100% B decreased to 90% over 2.5 min and to 50% over the next 5 min and returned to 100% over 0.5 min for 12 min of column re-equilibration. |
| Flow Rate: | 0.2 ml/min |
| Injection Temperature: | 4 |
| Solvent A: | 100% water; 10 mM ammonium bicarbonate |
| Solvent B: | 95% acetonitrile; 10 mM ammonium bicarbonate |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002902 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Phenomonex Kinetex C18 (50 x 2.1mm, 2.6um) |
| Column Temperature: | RT |
| Flow Gradient: | Mobile phase 100% A held for 1.25 min, then decreased to 50% over 1.75 min and to 0% over the next 0.5 min, held at 0% for 2 min and returned to 100% over 0.5 min for 4 min of column re-equilibration. |
| Flow Rate: | 0.3 ml/min |
| Injection Temperature: | 4 |
| Solvent A: | 100% water; 0.08% acetic acid |
| Solvent B: | 100% acetonitrile; 0.08% acetic acid |
| Chromatography Type: | Reversed phase |
