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MB Sample ID: SA244204
Local Sample ID: | SC_20201109_HILICp_FMS_CSF_F05_C_1_2 |
Subject ID: | SU002531 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003979 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE |
Units | time_m/z |
Chromatography:
Chromatography ID: | CH002942 |
Chromatography Summary: | CSF extracts (5uL injection volume) were separated on an ACQUITY UPLC BEH Amide HILIC 1.7μm, 2.1 × 100 mm column (Waters Corporation, Milford, MA) held at 30°C as previously described (cite). Briefly, liquid chromatography was performed at 200 µL min−1 using solvent A (5 mM ammonium formate in 90% water, 10% acetonitrile and 0.1% formic acid) and solvent B (5 mM ammonium formate in 90% acetonitrile, 10% water and 0.1% formic acid) with a gradient length of 30 min. |
Methods Filename: | Metabolomics_Analyses.pdf |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
Column Temperature: | 30 |
Flow Gradient: | 30 min |
Flow Rate: | 0.20mL/min |
Solvent A: | 90% water, 10% acetonitrile, 5mM Ammonium Formate, 0.1%FA |
Solvent B: | 10% water, 90% acetonitrile, 5mM Ammonium Formate, 0.1%FA |
Chromatography Type: | HILIC |