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MB Sample ID: SA244206
| Local Sample ID: | SC_20201109_HILICp_FMS_CSF_D02_C_1_1 |
| Subject ID: | SU002531 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003979 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | time_m/z |
Chromatography:
| Chromatography ID: | CH002942 |
| Chromatography Summary: | CSF extracts (5uL injection volume) were separated on an ACQUITY UPLC BEH Amide HILIC 1.7μm, 2.1 × 100 mm column (Waters Corporation, Milford, MA) held at 30°C as previously described (cite). Briefly, liquid chromatography was performed at 200 µL min−1 using solvent A (5 mM ammonium formate in 90% water, 10% acetonitrile and 0.1% formic acid) and solvent B (5 mM ammonium formate in 90% acetonitrile, 10% water and 0.1% formic acid) with a gradient length of 30 min. |
| Methods Filename: | Metabolomics_Analyses.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| Column Temperature: | 30 |
| Flow Gradient: | 30 min |
| Flow Rate: | 0.20mL/min |
| Solvent A: | 90% water, 10% acetonitrile, 5mM Ammonium Formate, 0.1%FA |
| Solvent B: | 10% water, 90% acetonitrile, 5mM Ammonium Formate, 0.1%FA |
| Chromatography Type: | HILIC |
