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MB Sample ID: SA254180

Local Sample ID:OC165
Subject ID:SU002621
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Combined analysis:

Analysis ID AN004153 AN004154
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Thermo Accucore C30 (150 x 2.1mm,2.6um) Thermo Accucore C30 (150 x 2.1mm,2.6um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Orbitrap ID-X tribrid Thermo Orbitrap ID-X tribrid
Ion Mode NEGATIVE POSITIVE
Units chromatographic peak area chromatographic peak area

Chromatography:

Chromatography ID:CH003073
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C30 (150 x 2.1mm,2.6um)
Column Temperature:50
Flow Gradient:0-1 min 80-40% A; 1-5 min 40-30% A; 5-5.5 min 30-15% A; 5.5-8 min 15-10% A; 8-8.2 min 10-0% A; 8.2-10.5 0% A; 10.5-10.7 min 0-80% A; 10.7-12.0 min 80% A.
Flow Rate:0.4 ml min-1
Solvent A:10 mM ammonium acetate with water/acetonitrile (40:60 v/v)
Solvent B:10 mM ammonium acetate with 2-isopropanol/acetonitrile (90:10 v/v). 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH003074
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C30 (150 x 2.1mm,2.6um)
Column Temperature:50
Flow Gradient:0-1 min 80-40% A; 1-5 min 40-30% A; 5-5.5 min 30-15% A; 5.5-8 min 15-10% A; 8-8.2 min 10-0% A; 8.2-10.5 0% A; 10.5-10.7 min 0-80% A; 10.7-12.0 min 80% A.
Flow Rate:0.4 ml min-1
Solvent A:10 mM ammonium formate with water/acetonitrile (40:60 v/v) and 0.1% formic acid
Solvent B:10 mM ammonium formate with 2-isopropanol/acetonitrile (90:10 v/v) and 0.1% formic acid
Chromatography Type:Reversed phase
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