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MB Sample ID: SA254503
| Local Sample ID: | MK115 |
| Subject ID: | SU002623 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 68 (63, 73) |
| Weight Or Weight Range: | 77 (62, 87) |
| Height Or Height Range: | 170 (160, 176) |
| Gender: | Male and female |
| Human Ethnicity: | White |
| Human Medications: | anti-PD-1 immune checkpoint inhibitors (nivolumab or pembrolizumab) |
| Human Inclusion Criteria: | consecutive advanced cancer patients |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004157 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | Thermo Orbitrap Exploris 240 |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003076 |
| Chromatography Summary: | The samples were analysed with our semi-polar metabolites method, which is a slightly modified version of the protocol described by Catalin et al. (UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes, Water Application note 2011, 720004042en). The analysis was carried out in a randomised order using a UPLC system (Vanquish, Thermo Fisher Scientific) coupled with a high-resolution quadrupole-orbitrap mass spectrometer Orbitrap Exploris 240 MS, Thermo Fisher Scientific. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Gradient: | 300 μL/min: 0-2 min: 0% B, 2-12 min: 35% B, 12-13 min: 90% B, 13-14 min: 90% B, 14-15 min: 0% B |
| Flow Rate: | 300 μL/min |
| Solvent A: | 100% water; 10 mM ammonium formate; 0.1% formic acid (pH 3.1) |
| Solvent B: | 100% methanol; 10 mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
