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MB Sample ID: SA255091

Local Sample ID:Purslane_SP3_Adult_Root_Polar_Negative
Subject ID:SU002637
Subject Type:Plant
Subject Species:Portulaca oleracea
Taxonomy ID:46147

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Combined analysis:

Analysis ID AN004175 AN004176
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu Nexera X2 Shimadzu Nexera X2
Column Waters ACQUITY UPLC BEH C8 (150 x 2.1mm,1.7um) Waters ACQUITY UPLC BEH C8 (150 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Bruker maXis Impact qTOF Bruker maXis Impact qTOF
Ion Mode POSITIVE NEGATIVE
Units m/z m/z

Chromatography:

Chromatography ID:CH003093
Instrument Name:Shimadzu Nexera X2
Column Name:Waters ACQUITY UPLC BEH C8 (150 x 2.1mm,1.7um)
Column Temperature:40 °C
Flow Gradient:isocratic at the start (0 - 0.5 min) with 4% of B solvent, then at linear gradient (0.5 – 10 min) with 34% B and (10 – 15 min) with 100% B, and finally isocratic (15 – 18 min) with 100% B
Flow Rate:400 μL min−1
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
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