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MB Sample ID: SA255618
| Local Sample ID: | m6 |
| Subject ID: | SU002643 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | MDA-MB-231 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004190 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | HP GCD 1800B |
| Column | Agilent PoraBOND Q (25m x 0.32mm x 0.5um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent/HP 5972 MSD |
| Ion Mode | POSITIVE |
| Units | pg/hr/ug and g/hr for media |
Chromatography:
| Chromatography ID: | CH003104 |
| Chromatography Summary: | Collected canister samples were transferred to a liquid nitrogen trap through pressure differential. Pressure change between beginning and end of “injection” was measured, allowing calculation of the moles of canister collected air injected. Sample in the trap was then transferred, via heated helium flow, to an Aglient/HP 5972 MSD system (Santa Clara, CA, USA) equipped with a PoraBond Q column (25 m x 0.32 mm x 0.5 μm film thickness) (Restek©, Bellefonte, PN, USA). Targeted samples were analyzed in selected ion monitoring (SIM) mode, and untargeted samples in full scan (SCAN) mode with the mass range of 45-200 amu. The mass spectrometer was operated in electron impact ionization mode with 70 eV ionization energy, and transfer line, ion source, and quadrupole temperatures of 250, 280 and 280, respectively. For details on SIM and significantly altered, identified SCAN compounds, see Table 1. All samples were analysed within 6 days of collection. The oven program for both SIM and SCAN analyses were identical and are as follows: 35 ˚C for 2 min, 10 ˚C/min to 155 ˚C, 1 ˚C/min to 131 ˚C and 25 ˚C/min to 250 with a 5 min 30 second hold. |
| Instrument Name: | HP GCD 1800B |
| Column Name: | Agilent PoraBOND Q (25m x 0.32mm x 0.5um) |
| Column Temperature: | 250 |
| Flow Gradient: | NA |
| Flow Rate: | 10ml/min |
| Solvent A: | NA |
| Solvent B: | NA |
| Chromatography Type: | GC |
