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MB Sample ID: SA256159
Local Sample ID: | B_Serum_43 |
Subject ID: | SU002650 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004198 | AN004199 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 | Agilent 1290 |
Column | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent Pursuit 5 C18 (150 x 2.1mm,5um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | POSITIVE | POSITIVE |
Units | nM | nM |
Chromatography:
Chromatography ID: | CH003111 |
Chromatography Summary: | LC-MS/MS (MRM) for phospholipid |
Instrument Name: | Agilent 1290 |
Column Name: | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) |
Column Temperature: | 30 |
Flow Gradient: | isocratic condition of 60% B |
Flow Rate: | 400 µL/min |
Solvent A: | 10 mM ammonium acetate in MeOH/isopropanol/H2O(900/50/50) |
Solvent B: | 10 mM ammonium acetate in MeOH/isopropanol/H2O(940/50/10) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003112 |
Chromatography Summary: | LC-MS/MS (MRM) for sphingolipid |
Instrument Name: | Agilent 1290 |
Column Name: | Agilent Pursuit 5 C18 (150 x 2.1mm,5um) |
Column Temperature: | 30 |
Flow Gradient: | 50% A for 0 min, 50% A for 5 min, 50 to 30% A for 3 min, 30% A for 7 min, 30 to 10% A for 7 min, 10% A for 3 min, 10 to 50% A for 0.1 min, and 50% A for 4.9 min |
Flow Rate: | 200 µL/min |
Solvent A: | 5 mM ammonium formate/MeOH/tetrahydrofuran(500/200/300) |
Solvent B: | 5 mM ammonium formate/MeOH/tetrahydrofuran(100/200/700) |
Chromatography Type: | Reversed phase |