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MB Sample ID: SA256485
| Local Sample ID: | BD11 |
| Subject ID: | SU002654 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 15-38 |
| Gender: | Male and female |
| Human Exclusion Criteria: | Subjects with other psychiatric or neurological disorders were excluded |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004205 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | UltiMate 3000 UHPLC system (Thermo Fisher Scientific) |
| Column | ACQUITY CSH C18 (2.1 ⨯ 100 mm, 1.7 μm) column (Waters) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE |
| Units | Intensity |
Chromatography:
| Chromatography ID: | CH003116 |
| Chromatography Summary: | Ultra-high performance liquid chromatography coupled to mass spectrometry (UHPLC-MS) analyses were performed on an UltiMate 3000 UHPLC system (Thermo Fisher Scientific, Waltham, MA, USA) coupled to a QExactive Orbitrap mass spectrometer (Thermo Fisher Scientific). The chromatographic separation was done on an ACQUITY CSH C18 (2.1 ⨯ 100 mm, 1.7 μm) column (Waters, Milford, MA, USA) and the temperature of the column oven was set to 55 ºC. The mobile phase A consisted of an ACN:water mixture (60:40) with 1 mmol/L ammonium formate and 0.1% (v/v) formic acid, and the mobile phase B consisted of an IPA:ACN mixture (90:10) with 1 mmol/L ammonium formate and 0.1% (v/v) formic acid. The flow rate was 0.4 mL/min and the injection volume was 5 μL for each sample. The gradient elution program consisted of a first linear gradient from solvent (A/B: 60/40) to solvent (A/B: 57/43) over 2 min; a rapid increase to solvent (A/B: 50/50); a second linear gradient to solvent (A/B: 46/54) over 10 min; a rapid increase to solvent (A/B: 30/70); a third linear gradient to solvent (A/B: 1/99) over 6 min; a rapid de-crease to solvent (A/B: 60/40); and finally, an isocratic elution of the solvent (A/B: 60/40) for 2 min. The column was equilibrated with solvent (A/B: 60/40) for 5 min before reuse. The total run time was 25 min for each analysis. Samples were randomized prior to analysis. To verify system stability, quality control (QC) samples were injected at the start and at the end of a run and after every 10th sample. |
| Instrument Name: | UltiMate 3000 UHPLC system (Thermo Fisher Scientific) |
| Column Name: | ACQUITY CSH C18 (2.1 ⨯ 100 mm, 1.7 μm) column (Waters) |
| Column Temperature: | 55 |
| Flow Gradient: | Gradient elution program (view Summary) |
| Flow Rate: | 0.4 mL/min |
| Sample Injection: | 5 μL |
| Solvent A: | 60% acetonitrile/40% water; 1 mM ammonium formate; 0.1% formic acid |
| Solvent B: | 90% isopropanol/10% acetonitrile; 1mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
