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MB Sample ID: SA258352
| Local Sample ID: | ap1calAP1GR_cytokinins_3 |
| Subject ID: | SU002672 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
| Genotype Strain: | ap1 cal AP1-GR and drmy1 ap1 cal AP1-GR |
| Age Or Age Range: | Bolting (40-50 days after germination) |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004236 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003143 |
| Chromatography Summary: | 1 µl of each sample was injected into a Thermo Fisher Scientific Vanquish Horizon UHPLC System coupled with a Thermo Q Exactive HF hybrid quadropole-orbitrap high-resolution mass spectrometer equipped with a HESI ion source. Samples were separated on a C18 ODS column (AQUITY UPLC BEH C18, 1.7 μm, 2.1 × 100 mm, Waters), at a flow rate of 0.3 ml/min, with linear gradients of solvent A (0.1% formic acid) and solvent B (0.1% formic acid in methanol) according to the following profile: 0 min, 99.0% A + 1.0% B; 4.0 min, 55.0% A + 45.0% B; 7 min, 30.0% A + 70.0% B; and then with isocratic conditions: 8 min, 1.0% A + 99.0% B; 12 min, 99.0% A + 1.0% B. |
| Methods Filename: | Protocol_SK.PDF |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 60 °C |
| Flow Gradient: | 0 min, 99.0% A + 1.0% B; 4.0 min, 55.0% A + 45.0% B; 7 min, 30.0% A + 70.0% B; and then with isocratic conditions: 8 min, 1.0% A + 99.0% B; 12 min, 99.0% A + 1.0% B |
| Flow Rate: | 0.3 ml/min |
| Internal Standard: | BAP |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Preconditioning: | Mili-Q H2O |
| Chromatography Type: | Reversed phase |
