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MB Sample ID: SA258369
Local Sample ID: | PTEN WT Rep3 |
Subject ID: | SU002674 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | embryonic fibroblasts |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004238 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Waters Acquity |
Column | Waters XBridge Amide (100 x 4.6mm,3.5um) |
MS Type | APCI |
MS instrument type | QTRAP |
MS instrument name | ABI Sciex 5500 QTrap |
Ion Mode | POSITIVE |
Units | peak area intensity |
Chromatography:
Chromatography ID: | CH003145 |
Instrument Name: | Waters Acquity |
Column Name: | Waters XBridge Amide (100 x 4.6mm,3.5um) |
Column Temperature: | 45 |
Flow Gradient: | 85% buffer B to 30% buffer B from 0 - 3 minutes, then 30% buffer B to 3% buffer B from minute 3 to 12, then 2% buffer B held from minute 12 to 15, followed by 2% buffer B to 85% buffer B from minute 15 to 16, then 85% buffer B held from minute 16-23 in order to re-equilibrate the column. |
Flow Rate: | 400uL/minute |
Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium hydroxide; 20 mM ammonium acetate |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |