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MB Sample ID: SA260966
| Local Sample ID: | 90225016811 |
| Subject ID: | SU002716 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004286 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity UPLC |
| Column | Waters Acquity UPLC BEH C18 (50 x 2.1 mm,1.7 um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Xevo TQ-S |
| Ion Mode | POSITIVE |
| Units | pmol/mg of tissue |
Chromatography:
| Chromatography ID: | CH003192 |
| Chromatography Summary: | The analytical column is used at 30°C. 10 µl of the sample are injected onto the column and eluted at a flow rate of 0.4 ml/min. The gradient begins with 45% eluent A (5 mM ammonium acetate in water) and is then programmed as follows: 0 to 2 min – gradient to 55% eluent B (methanol); 2 to 2.5 min - gradient to 95% eluent B; 2.5 to 3.2 min - hold at 95% eluent B, return to 45% A, and re-equilibrate the column at initial conditions for 1 minute. |
| Methods Filename: | pass1b_ka_methods.pdf |
| Instrument Name: | Waters Acquity UPLC |
| Column Name: | Waters Acquity UPLC BEH C18 (50 x 2.1 mm,1.7 um) |
| Column Temperature: | 30C |
| Flow Gradient: | 45% A and 55% B for 2 minutes, followed by a linear gradient to 95% B from 2 to 2.5 minutes, held at 95% B for 0.7 minutes, returned to 45% A, and finally the column was re-equilibrated at initial conditions for 1 minute. The total run time was 4.7 minutes. |
| Flow Rate: | 0.4 ml/min |
| Solvent A: | 100% water; 5 mM ammonium acetate |
| Solvent B: | 100% methanol |
| Chromatography Type: | Reversed phase |
