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MB Sample ID: SA260982
Local Sample ID: | 80011826802_4 |
Subject ID: | SU002717 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004287 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity UPLC |
Column | Chromolith FastGradient RP-18e 50-2mm column (EMD Millipore, Billerica, MA, USA) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Waters Xevo TQ-XS |
Ion Mode | NEGATIVE |
Units | pmol/mg of tissue |
Chromatography:
Chromatography ID: | CH003193 |
Chromatography Summary: | Injection volume is 2 ”L. Mobile phase A is 95% water, 5% methanol and 5 mM dimethylhexylamine adjusted to pH 7.5 with acetic acid. Mobile phase B is 20% water, 80% methanol and 10 mM dimethylhexylamine. Flow rate is set to 0.3 mL/min and column temperature is 40 °C. A 22 min gradient method (t=0, %B=0; t=1.2, %B=0; t=22, %B=40) is run followed by a 3 min wash and 7 min equilibration. |
Methods Filename: | pass1b_nuc_methods.pdf |
Instrument Name: | Waters Acquity UPLC |
Column Name: | Chromolith FastGradient RP-18e 50-2mm column (EMD Millipore, Billerica, MA, USA) |
Column Temperature: | 40C |
Flow Gradient: | 22-minute gradient (t=0, %B=0; t=1.2, %B=0; t=22, %B=40) followed by a 3-minute wash and 7-minute equilibration |
Flow Rate: | 0.3 ml/min |
Solvent A: | 95% water/5% methanol; 5 mM dimethylhexylamine adjusted to pH 7.5 with acetic acid |
Solvent B: | 20% water/80% methanol; 10 mM dimethylhexylamine |
Chromatography Type: | Reversed phase |