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MB Sample ID: SA271097
| Local Sample ID: | C_Serum_70_EnergyMetabolism |
| Subject ID: | SU002807 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004379 | AN004380 | AN004381 | AN004382 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | GC |
| Chromatography system | Agilent 1290 | Agilent 1290 | Thermo Dionex Ultimate 3000 | Agilent 7890A |
| Column | Phenomenex Synergi Fusion-RP (100 x 4.6mm,4um) | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) | Phenomenex Jupiter C18 (150 x 2mm,5um) | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | ESI | ESI | ESI | EI |
| MS instrument type | QTRAP | QTRAP | Orbitrap | Single quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | Thermo LTQ XL | Agilent 5975C |
| Ion Mode | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE |
| Units | nM | uM | ug/uL | area ratio |
Chromatography:
| Chromatography ID: | CH003284 |
| Chromatography Summary: | Metabolites related to energy metabolism |
| Instrument Name: | Agilent 1290 |
| Column Name: | Phenomenex Synergi Fusion-RP (100 x 4.6mm,4um) |
| Column Temperature: | 23 |
| Flow Gradient: | Hold at 0% B for 5 min, 0% to 90% B for 2 min, hold at 90% for 8 min, 90% to 0% B for 1 min, then hold at 0% B for 9 min |
| Flow Rate: | 70 μL/min except for 140 μL/min during minutes 7–15 |
| Solvent A: | 100% water; 5mM ammonium acetate |
| Solvent B: | 100% methanol; 5 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003285 |
| Chromatography Summary: | Amino acids |
| Instrument Name: | Agilent 1290 |
| Column Name: | Agilent ZORBAX Eclipse Plus C18 (50 x 2.1mm,1.8um) |
| Column Temperature: | 50 |
| Flow Gradient: | Hold at 0% B for 0.5 min, 0–95% B for 5 min, 95% B for 1 min, and 95–0% B for 0.5 min, then hold at 0% B for 2.5 min |
| Flow Rate: | 500 μL/min |
| Solvent A: | 100% water; 0.2% formic acid |
| Solvent B: | 100% acetonitrile; 0.2% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003286 |
| Chromatography Summary: | S1P |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Phenomenex Jupiter C18 (150 x 2mm,5um) |
| Column Temperature: | 35°C |
| Flow Gradient: | 10% B for 0 min, 10–90% B for 5 min, 90% B for 10 min, 90–10% B for 0.1 min, and 10% B for 4.9 min |
| Flow Rate: | 300 μL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003287 |
| Chromatography Summary: | Free fatty acids (FFA) |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| Column Temperature: | - |
| Flow Gradient: | - |
| Flow Rate: | - |
| Solvent A: | - |
| Solvent B: | - |
| Chromatography Type: | GC |
