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MB Sample ID: SA272176
| Local Sample ID: | PL11 |
| Subject ID: | SU002811 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | B6.129-Arntltm1Bra/J |
| Age Or Age Range: | 40 weeks |
| Gender: | Male |
| Animal Feed: | Ad libitum |
| Animal Water: | Ad libitum |
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Combined analysis:
| Analysis ID | AN004387 | AN004388 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex | Thermo Dionex |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm,2um) | ACE Excel 2 C18-PFP (100 x 2.1mm,2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak height | Peak height |
Chromatography:
| Chromatography ID: | CH003291 |
| Chromatography Summary: | Global metabolomics profiling was performed on a Thermo Q-Exactive Oribtrap mass spectrometer with Dionex UHPLC and autosampler. Each extract was spiked with 1 ul of standard mixture consisting of deuterium labeled carnitine, acetylcarnitine, propionylcarnitine, butyrylcarnitine, isovalerylcarnitine, octanoylcarnitine, myristoylcarnitine and palmitoylcarnitine to targeted identification of carnitines in the sample. All samples were analyzed in positive and negative heated electrospray ionization with a mass resolution of 35,000 at m/z 200 as separate injections. Separation was achieved on an ACE 2 Excel-C18 pfp, 100x2.1mm, 2um column with mobile phase A as 0.1% formic acid in water and mobile phase B as acetonitrile. This is a polar embedded stationary phase that provides comprehensive coverage, but does have some limitation is the coverage of very polar species. The flow rate was 350 µL/min with a column temperature of 25°C. 4 µL was injected for negative ions and 2 µL for positive ions. |
| Instrument Name: | Thermo Dionex |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm,2um) |
| Column Temperature: | 25°C |
| Flow Gradient: | - |
| Flow Rate: | 350 µL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
| Solvent C: | - |
