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MB Sample ID: SA272221

Local Sample ID:RF150
Subject ID:SU002812
Subject Type:Mammal
Subject Species:Mus musculus

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Combined analysis:

Analysis ID AN004389
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Ascentis Express C18 (150 x 2.1mm,2.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE
Units nM

Chromatography:

Chromatography ID:CH003292
Chromatography Summary:Ascentis Express C18 HPLC column (2.7 μm × 15 cm × 2.1 mm; Sigma Aldrich).
Instrument Name:Thermo Vanquish
Column Name:Ascentis Express C18 (150 x 2.1mm,2.7um)
Column Temperature:30
Flow Gradient:0–5 min: gradient was held at 5% B; 2–12.1 min: linear gradient of 5% to 95% B; 12.1–17.0 min: 95% B; 17.1–21.0 min: gradient was returned to 5% B
Flow Rate:0.250 ml/min
Solvent A:water, 0.1% formic acid
Solvent B:acetonitrile, 0.1% formic acid
Chromatography Type:Reversed phase
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