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MB Sample ID: SA273536
Local Sample ID: | A375 VR 2 |
Subject ID: | SU002818 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Gender: | Female |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | A375 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004397 | AN004398 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | NEGATIVE | POSITIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH003299 |
Chromatography Summary: | Negative C18 |
Instrument Name: | Thermo Vanquish |
Column Name: | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) |
Column Temperature: | 45 |
Flow Gradient: | 0-3 min 10-95% B at 0.3 mL/min, 3-4.2 min hold at 95% B at 0.3 mL/min, 4.2-4.3 min drop to 10% B at 0.45 mL/min, 4.3-4.9 min hold at 10%B while lowering flow rate to 0.4 mL/min, 4.9-5 min hold at 10%B while lowering flow rate to 0.3 mL/min. |
Flow Rate: | see gradient |
Sample Injection: | 10 uL |
Solvent A: | 75% water 25% acetonitrile 5 mM ammonium acetate |
Solvent B: | 90% isopropanol 10% acetonitrile 5 mM ammonium acetate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003300 |
Chromatography Summary: | Positive C18 |
Instrument Name: | Thermo Vanquish |
Column Name: | Phenomenex Kinetex C18 (30 x 2.1mm,1.7um) |
Column Temperature: | 45 |
Flow Gradient: | 0-3 min 30-100% B at 0.3 mL/min, 3-4.2 min hold at 100% B at 0.3 mL/min, 4.2-4.3 min 100-30% B at 0.4 mL/min, 4.3-4.9 min hold at 30%B and 0.4 mL/min, 4.9-5 min hold at 30%B while lowering flow rate to 0.3 mL/min |
Flow Rate: | see gradient |
Sample Injection: | 10 uL |
Solvent A: | 75% water 25% acetonitrile 5 mM ammonium acetate |
Solvent B: | 90% isopropanol 10% acetonitrile 5 mM ammonium acetate |
Chromatography Type: | Reversed phase |