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MB Sample ID: SA289187
Local Sample ID: | MED1 PO Condensate Sample 5 |
Subject ID: | SU002852 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Combined analysis:
Analysis ID | AN004451 | AN004452 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent Model 1290 Infinity II liquid chromatography system | Agilent Model 1290 Infinity II liquid chromatography system |
Column | Cogent Diamond Hydride (150 × 2.1 mm, 4um) | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6460 QQQ | Agilent 6460 QQQ |
Ion Mode | POSITIVE | POSITIVE |
Units | Ion abundance (peak area) | Ion abundance (peak area) |
Chromatography:
Chromatography ID: | CH003343 |
Chromatography Summary: | Chromatography of metabolites utilized reversed phase chromatography on a Agilent ZORBAX Eclipse Plus C18, 100 × 2.1 mm, 1.8 μm. Mobile phases consisted of (A) 10 mM ammonium formate with 5 μM Agilent deactivator additive in 5:3:2 water:acetonitrile:2-propanol and (B) 10 mM ammonium formate in 1:9:90 water:acetonitrile:2-propanol. Column temperature was set at 60°C and autosampler temperature was at 20°C. The flow rate was 0.4 mL/min. The following gradient was applied: 0 min, 15% B; 0-2.5 min, to 50% B; 2.5-2.6 min, to 57%, 2.6-9 min, to 70% B; 9-9.1 min, to 93% B; 9.1-11.1 min, to 96%; 11.1- 15min, 100% B; 15-20 min, 15% B. |
Instrument Name: | Agilent Model 1290 Infinity II liquid chromatography system |
Column Name: | Cogent Diamond Hydride (150 × 2.1 mm, 4um) |
Column Temperature: | 60 |
Flow Gradient: | 0 min, 15% B; 0-2.5 min, to 50% B; 2.5-2.6 min, to 57%, 2.6-9 min, to 70% B; 9-9.1 min, to 93% B; 9.1-11.1 min, to 96%; 11.1- 15min, 100% B; 15-20 min, 15% B. |
Flow Rate: | 0.4 mL/min |
Solvent A: | 50% water/30% acetonitrile/20% isopropanol;10 mM ammonium formate with 5 µM Agilent deactivator additive |
Solvent B: | 1% water/9% acetonitrile/90% isopropanol;10 mM ammonium formate |
Chromatography Type: | Reversed phase |