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MB Sample ID: SA289308
Local Sample ID: | 407 661 |
Subject ID: | SU002855 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 8-weeks old |
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Combined analysis:
Analysis ID | AN004458 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo-Fisher Ultimate 3000 HPLC |
Column | Agilent Poroshell 120 HILIC-Z |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6600 QTOF |
Ion Mode | NEGATIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH003347 |
Chromatography Summary: | Liquid chromatography-mass spectrometry analysis was performed using a Thermo-Fisher Ultimate 3000 HPLC system consisting of an HPG-3400RS high pressure gradient pump, TCC 3000SD column compartment and WPS 3000 Autosampler, coupled to a SCIEX 6600 TripleTOF Q-TOF mass spectrometer with TurboV ion source. The system was controlled by SCIEX Analyst 1.7.1, DCMS Link and Chromeleon Xpress software. |
Instrument Name: | Thermo-Fisher Ultimate 3000 HPLC |
Column Name: | Agilent Poroshell 120 HILIC-Z |
Column Temperature: | 40 |
Flow Gradient: | flow rate of 0.25 ml/min, starting at 96 % B for 2 minutes, ramping to 65 % B over 20 min, hold at 65 % B for 2 min, then back to 96 % B |
Flow Rate: | 0.25 ml/min |
Solvent A: | 100% water; 10 mM ammonium acetate adjusted to pH 9 with ammonium hydroxide and 20 µM medronic acid |
Solvent B: | 85% acetonitrile/15% water; 10 mM ammonium acetate adjusted to pH 9 with ammonium hydroxide and 20 µM medronic acid |
Chromatography Type: | HILIC |