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MB Sample ID: SA289310
| Local Sample ID: | 407 660 |
| Subject ID: | SU002855 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 8-weeks old |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004458 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo-Fisher Ultimate 3000 HPLC |
| Column | Agilent Poroshell 120 HILIC-Z |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6600 QTOF |
| Ion Mode | NEGATIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003347 |
| Chromatography Summary: | Liquid chromatography-mass spectrometry analysis was performed using a Thermo-Fisher Ultimate 3000 HPLC system consisting of an HPG-3400RS high pressure gradient pump, TCC 3000SD column compartment and WPS 3000 Autosampler, coupled to a SCIEX 6600 TripleTOF Q-TOF mass spectrometer with TurboV ion source. The system was controlled by SCIEX Analyst 1.7.1, DCMS Link and Chromeleon Xpress software. |
| Instrument Name: | Thermo-Fisher Ultimate 3000 HPLC |
| Column Name: | Agilent Poroshell 120 HILIC-Z |
| Column Temperature: | 40 |
| Flow Gradient: | flow rate of 0.25 ml/min, starting at 96 % B for 2 minutes, ramping to 65 % B over 20 min, hold at 65 % B for 2 min, then back to 96 % B |
| Flow Rate: | 0.25 ml/min |
| Solvent A: | 100% water; 10 mM ammonium acetate adjusted to pH 9 with ammonium hydroxide and 20 µM medronic acid |
| Solvent B: | 85% acetonitrile/15% water; 10 mM ammonium acetate adjusted to pH 9 with ammonium hydroxide and 20 µM medronic acid |
| Chromatography Type: | HILIC |
