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MB Sample ID: SA290901
Local Sample ID: | 20220113_Cowley3_OM_14_2-466_HILICpos_rep |
Subject ID: | SU002867 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
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Combined analysis:
Analysis ID | AN004483 | AN004484 | AN004485 | AN004486 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
Column | Agilent InfinityLab Poroshell 120 EC-C18 (2.1 x 50 mm; 2.7-Micron) | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) | Agilent InfiinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Area | Area | Area | Area |
Chromatography:
Chromatography ID: | CH003368 |
Chromatography Summary: | This chromatography method was utilized for all C18 positive polarity runs in this study. |
Instrument Name: | Thermo Vanquish |
Column Name: | Agilent InfinityLab Poroshell 120 EC-C18 (2.1 x 50 mm; 2.7-Micron) |
Column Temperature: | 25C |
Flow Gradient: | 0-1 minutes at 98% A1/2% B1, 1-13 minutes from 98% A1/2% B1 to 10% A1/90% B1, 13-15 minutes at 10% A1/90% B1, 15-16 minutes from 10% A1/90% B1 to 98% A1/2% B1, and was re-equilibrated from 16-25 minutes at 98% A1/2% B1 |
Flow Rate: | 0.1 mL/minute |
Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
Solvent A: | 100% water, 0.2% acetic acid |
Solvent B: | 100% acetonitrile, 0.2% acetic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003369 |
Chromatography Summary: | This chromatography method was utilized for all C18 negative polarity runs in this study. |
Instrument Name: | Thermo Vanquish |
Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
Column Temperature: | 25C |
Flow Gradient: | 0-1 minutes at 2% A/98% B, 1-11 minutes from 2% A/98% B to 30% A/70% B, 11-12 minutes from 30% A/70% B to 40% A/60% B, 12-16 minutes from 40% A/60% B to 95% A/5% B, was held at 95% A/5% B from 16-18 minutes, 18-20 minutes from 95% A/5% B to 2% A/98% B, and was re-equilibrated from 20-25 minutes at 2% A/98% B |
Flow Rate: | 0.1 mL/minute |
Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
Solvent A: | 100% water, 0.2% acetic acid |
Solvent B: | 100% acetonitrile, 0.2% acetic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003370 |
Chromatography Summary: | This chromatography method was utilized for all HILIC positive polarity runs in this study. |
Instrument Name: | Thermo Vanquish |
Column Name: | Agilent InfinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
Column Temperature: | 25C |
Flow Gradient: | 0-1 minutes at 2% A/98% B, 1-11 minutes from 2% A/98% B to 30% A/70% B, 11-12 minutes from 30% A/70% B to 40% A/60% B, 12-16 minutes from 40% A/60% B to 95% A/5% B, was held at 95% A/5% B from 16-18 minutes, 18-20 minutes from 95% A/5% B to 2% A/98% B, and was re-equilibrated from 20-25 minutes at 2% A/98% B |
Flow Rate: | 0.1 mL/minute |
Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
Solvent A: | 10 mM ammonium formate in H2O with 0.1% formic acid (Solvent A2) |
Solvent B: | 90% ACN with 10 mM ammonium formate in H2O with 0.1% formic acid (Solvent B2) |
Chromatography Type: | HILIC |
Chromatography ID: | CH003371 |
Chromatography Summary: | This chromatography method was utilized for all HILIC negative polarity runs in this study. |
Instrument Name: | Thermo Vanquish |
Column Name: | Agilent InfiinityLab Poroshell 120 HILIC-Z (2.1 x 50 mm; 2.7 micron; #689775-924) |
Column Temperature: | 25C |
Flow Gradient: | 0-1 minutes at 2% A/98% B, 1-11 minutes from 2% A/98% B to 30% A/70% B, 11-12 minutes from 30% A/70% B to 40% A/60% B, 12-16 minutes from 40% A/60% B to 95% A/5% B, was held at 95% A/5% B from 16-18 minutes, 18-20 minutes from 95% A/5% B to 2% A/98% B, and was re-equilibrated from 20-25 minutes at 2% A/98% B |
Flow Rate: | 0.1 mL/minute |
Internal Standard: | Caffeine, 1-napthylamine, and 9-anthracene carboxylic acid were all added at 0.5 ng/ µL in the extraction buffer as internal standards |
Solvent A: | 10 mM ammonium acetate in H2O, pH 9.0 with 0.1% AffinityLab Deactivator Inhibitor (Agilent, #5191-3940; Solvent A3) |
Solvent B: | 85% ACN with 10 mM ammonium acetate in H2O with 0.1% AffinityLab Deactivator Inhibitor (Solvent B3) |
Chromatography Type: | HILIC |