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MB Sample ID: SA294102
Local Sample ID: | INSPIRE_S_541 |
Subject ID: | SU002876 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004506 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Scientific Vanquish UPHPLC |
Column | Acquity UPLC HSS T3 C18 (100 X 2.1mm, 1.8um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF-X Orbitrap |
Ion Mode | POSITIVE |
Units | Normalized Intensities |
Chromatography:
Chromatography ID: | CH003386 |
Chromatography Summary: | Reversed phase |
Instrument Name: | Thermo Scientific Vanquish UPHPLC |
Column Name: | Acquity UPLC HSS T3 C18 (100 X 2.1mm, 1.8um) |
Column Pressure: | 6000-10000 psi |
Column Temperature: | 50 ℃ |
Flow Gradient: | Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5 |
Flow Rate: | 0.4 mL/min |
Injection Temperature: | 8 ℃ |
Internal Standard: | Tryptophan-d5 |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Analytical Time: | 22 min |
Capillary Voltage: | 3.5 KV |
Weak Wash Solvent Name: | 10% methanol/90% water; 0.1% formic acid |
Strong Wash Solvent Name: | 75% 2-Propanol/25% Water; 0.1% formic acid |
Chromatography Type: | Reversed phase |