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MB Sample ID: SA301588
Local Sample ID: | MUSC-00289 |
Subject ID: | SU002916 |
Subject Type: | Mammal |
Subject Species: | Mouse/canine |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004566 | AN004567 | AN004568 | AN004569 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | HILIC |
Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
Column | Waters Acquity BEH C18 (100 x 2mm, 1.7um) | Waters Acquity BEH C18 (100 x 2mm, 1.7um) | Waters Acquity BEH C18 (100 x 2mm, 1.7um) | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | POSITIVE | NEGATIVE | NEGATIVE |
Units | Normalized/scaled raw area counts | Normalized/scaled raw area counts | Normalized/scaled raw area counts | Normalized/scaled raw area counts |
Chromatography:
Chromatography ID: | CH003431 |
Chromatography Summary: | Low pH polar |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2mm, 1.7um) |
Column Temperature: | 65 |
Flow Gradient: | Linear gradient from 5% B to 80% B over 3.35 minutes. |
Flow Rate: | 0.35 mL/min |
Solvent A: | 0.1% formic acid and 0.05% PFPA in water, pH ~2.5 |
Solvent B: | 0.1% formic acid and 0.05% PFPA in methanol, pH ~2.5 |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003432 |
Chromatography Summary: | Low pH Lipophilic |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2mm, 1.7um) |
Column Temperature: | 65 |
Flow Gradient: | Linear gradient from 40 % B to 99.5% B over 1.0 minute, hold 99.5% B for 2.4 minutes |
Flow Rate: | 0.60 mL/min |
Solvent A: | 0.1% formic acid and 0.05% PFPA in water, pH ~2.5 |
Solvent B: | 0.1% formic acid and 0.05% PFPA in 50% methanol/ 50% acetonitrile, pH ~2.5 |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003433 |
Chromatography Summary: | High pH |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (100 x 2mm, 1.7um) |
Column Temperature: | 65 |
Flow Gradient: | Linear gradient from 0.5 to 70% B over 4.0 minutes, then rapid gradient to 99%B in 0.5 minutes. |
Flow Rate: | 0.35 mL/min |
Solvent A: | 6.5 mM ammonium bicarbonate in water, pH 8 |
Solvent B: | 6.5 mM ammonium bicarbonate in 95% methanol/ 5% water |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003434 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) |
Column Temperature: | 65 |
Flow Gradient: | Linear gradient from 5% B to 50% B in 3.5 minutes, then linear gradient from 50% B to 95% B in 2 minutes minutes. |
Flow Rate: | 0.50 mL/min |
Solvent A: | 10 mM ammonium formate in 15% water/ 5% methanol/ 80% acetonitrile (effective pH 10.16 with NH4OH) |
Solvent B: | 10 mM ammonium formate in 50% water/ 50% acetonitrile (effective pH 10.60 with NH4OH) |
Chromatography Type: | HILIC |