Return to study ST002851 main page
MB Sample ID: SA308633
Local Sample ID: | WT_Liver_MR07 |
Subject ID: | SU002963 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004670 | AN004671 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Agilent 1290 Infinity II Bio LC system | Agilent 1290 Infinity II Bio LC system |
Column | Agilent AdvanceBio MS Spent Media (150 x 2.1mm, 2.7um) | Agilent AdvanceBio MS Spent Media (150 x 2.1mm, 2.7um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Area | Peak Area |
Chromatography:
Chromatography ID: | CH003515 |
Chromatography Summary: | For liquid chromatography, an Agilent 1290 Infinity II Bio LC system (Agilent Technologies Inc., Waldbronn, Germany) consisting of a 1290 Bio High-Speed Pump, a 1290 Bio Multisampler and a 1290 MCT with an AdvanceBio MS Spent Media (150 x 2.1 mm, 2.7 µm; Agilent Technologies Inc., Waldbronn, Germany) were used. The elution was carried out by utilizing a gradient at a flow rate of 250 μL/min with water (10 mM ammonium acetate pH 9) as solvent A and acetonitrile/water (90/10; v/v; 10 mM ammonium acetate pH 9) as solvent B. The linear gradient was: 0 min, 90% B; 2 min, 90% B; 12 min, 40% B; 13 min, 20% B; 16 min, 20% B; followed by 9 min at initial condition for re-equilibration. Column temperature was 35 °C, injection volume 5 µL. |
Instrument Name: | Agilent 1290 Infinity II Bio LC system |
Column Name: | Agilent AdvanceBio MS Spent Media (150 x 2.1mm, 2.7um) |
Column Temperature: | 35 °C |
Flow Gradient: | none |
Flow Rate: | 250 μL/min |
Solvent A: | water (10 mM ammonium acetate pH 9) |
Solvent B: | acetonitrile/water (90/10; v/v; 10 mM ammonium acetate pH 9) |
Chromatography Type: | HILIC |