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MB Sample ID: SA308641

Local Sample ID:WT_Liver_MR08
Subject ID:SU002963
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN004670 AN004671
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1290 Infinity II Bio LC system Agilent 1290 Infinity II Bio LC system
Column Agilent AdvanceBio MS Spent Media (150 x 2.1mm, 2.7um) Agilent AdvanceBio MS Spent Media (150 x 2.1mm, 2.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak Area Peak Area

Chromatography:

Chromatography ID:CH003515
Chromatography Summary:For liquid chromatography, an Agilent 1290 Infinity II Bio LC system (Agilent Technologies Inc., Waldbronn, Germany) consisting of a 1290 Bio High-Speed Pump, a 1290 Bio Multisampler and a 1290 MCT with an AdvanceBio MS Spent Media (150 x 2.1 mm, 2.7 µm; Agilent Technologies Inc., Waldbronn, Germany) were used. The elution was carried out by utilizing a gradient at a flow rate of 250 μL/min with water (10 mM ammonium acetate pH 9) as solvent A and acetonitrile/water (90/10; v/v; 10 mM ammonium acetate pH 9) as solvent B. The linear gradient was: 0 min, 90% B; 2 min, 90% B; 12 min, 40% B; 13 min, 20% B; 16 min, 20% B; followed by 9 min at initial condition for re-equilibration. Column temperature was 35 °C, injection volume 5 µL.
Instrument Name:Agilent 1290 Infinity II Bio LC system
Column Name:Agilent AdvanceBio MS Spent Media (150 x 2.1mm, 2.7um)
Column Temperature:35 °C
Flow Gradient:none
Flow Rate:250 μL/min
Solvent A:water (10 mM ammonium acetate pH 9)
Solvent B:acetonitrile/water (90/10; v/v; 10 mM ammonium acetate pH 9)
Chromatography Type:HILIC
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