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MB Sample ID: SA315391
Local Sample ID: | H-Glu/75_repeat_2-neg |
Subject ID: | SU003003 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Genotype Strain: | BL21(DE3) |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004748 | AN004749 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | In-house packed nano column (ID 100 μm, Kinetex 1.7 μm C18 (100 Å) [Phenomenex], 12-15 cm. | In-house packed nano column (ID 100 μm, Kinetex 1.7 μm C18 (100 Å) [Phenomenex], 12-15 cm. |
MS Type | ESI | ESI |
MS instrument type | LTQ-FT | LTQ-FT |
MS instrument name | Thermo Velos Pro Orbitrap | Thermo Velos Pro Orbitrap |
Ion Mode | NEGATIVE | POSITIVE |
Units | Presence/Absence | Presence/Absence |
Chromatography:
Chromatography ID: | CH003581 |
Chromatography Summary: | Because the columns were packed in-house, there is some variation in the total length (see column description below). |
Methods Filename: | Deuterated_Lipids_Methods_Summary.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | In-house packed nano column (ID 100 μm, Kinetex 1.7 μm C18 (100 Å) [Phenomenex], 12-15 cm. |
Column Temperature: | room temp |
Flow Gradient: | Solvent Gradient: Time (min) 0 1 2.5 7.5 13.5 48.5 58.5 75.5 77.5 87 Solvent A (%) 99 99 70 65 45 30 1 1 99 99 Solvent B (%) 1 1 30 45 55 70 99 99 1 1 |
Flow Rate: | Split flow nano-chromatography. The actual flow rate at the column is dependent on a variety of factors and an accurate measurement of the flow rate is impractical. The flow rate at the macro pumps were set at either 0.05 or 0.1 mL/min depending on the waste line's backpressure. |
Solvent A: | 60% acetonitrile/40% water; ~0.4g/L ammonium acetate |
Solvent B: | 90% sopropanol/10% acetonitrile; ~0.4g/L ammonium acetate |
Chromatography Type: | Reversed phase |