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MB Sample ID: SA315462
Local Sample ID: | D1_2 |
Subject ID: | SU003004 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 15-20 week old |
Gender: | Male |
Species Group: | C57BL/6J |
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Combined analysis:
Analysis ID | AN004750 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Vanquish UHPLC |
Column | ACQUITY UPLC BEH Amide HILIC |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | NEGATIVE |
Units | time_m/z |
Chromatography:
Chromatography ID: | CH003582 |
Chromatography Summary: | Metabolite extracts were separated on ACQUITY UPLC BEH Amide HILIC 1.7 μm, 2.1 × 100 mm column (Waters Corporation, Milford, MA) held at 30°C. Liquid chromatography was performed at a 200 μL min using solvent A (5 mM Ammonium formate in 90% water, 10% acetonitrile, and 0.1% formic acid) and solvent B (5 mM Ammonium formate in 90% acetonitrile, 10% water, and 0.1% formic acid) with a gradient length of 30 min. |
Instrument Name: | Vanquish UHPLC |
Column Name: | ACQUITY UPLC BEH Amide HILIC |
Column Temperature: | 30 |
Flow Gradient: | Linear gradient of 30 min |
Flow Rate: | 0.20mL/min |
Solvent A: | 90% water/10% acetonitrile; 5mM ammonium formate; 0.1% formic acid |
Solvent B: | 10% water/90% acetonitrile; 5mM ammonium formate; 0.1% formic acid |
Chromatography Type: | HILIC |