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MB Sample ID: SA315468
| Local Sample ID: | D3_4 |
| Subject ID: | SU003004 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 15-20 week old |
| Gender: | Male |
| Species Group: | C57BL/6J |
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Combined analysis:
| Analysis ID | AN004750 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Vanquish UHPLC |
| Column | ACQUITY UPLC BEH Amide HILIC |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | NEGATIVE |
| Units | time_m/z |
Chromatography:
| Chromatography ID: | CH003582 |
| Chromatography Summary: | Metabolite extracts were separated on ACQUITY UPLC BEH Amide HILIC 1.7 μm, 2.1 × 100 mm column (Waters Corporation, Milford, MA) held at 30°C. Liquid chromatography was performed at a 200 μL min using solvent A (5 mM Ammonium formate in 90% water, 10% acetonitrile, and 0.1% formic acid) and solvent B (5 mM Ammonium formate in 90% acetonitrile, 10% water, and 0.1% formic acid) with a gradient length of 30 min. |
| Instrument Name: | Vanquish UHPLC |
| Column Name: | ACQUITY UPLC BEH Amide HILIC |
| Column Temperature: | 30 |
| Flow Gradient: | Linear gradient of 30 min |
| Flow Rate: | 0.20mL/min |
| Solvent A: | 90% water/10% acetonitrile; 5mM ammonium formate; 0.1% formic acid |
| Solvent B: | 10% water/90% acetonitrile; 5mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | HILIC |
