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MB Sample ID: SA315764
Local Sample ID: | 694 |
Subject ID: | SU003016 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 22-93 |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004762 | AN004763 | AN004764 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | HILIC | HILIC | Reversed phase |
Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
Column | Waters Atlantis HILIC (150 x 2.1mm,3um,100Å) | Phenomenex Luna NH2 (150 x 2 mm,5um,100Å) | Waters ACQUITY UPLC BEH C8 (2.1 mm X 100 mm,1.7 µm,130Å) |
MS Type | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE |
Units | integrated peak area | integrated peak area | integrated peak area |
Chromatography:
Chromatography ID: | CH003594 |
Chromatography Summary: | HILIC-pos: Hydrophilic interaction liquid chromatography (HILIC) analysis of water soluble metabolites in the positive ionization mode |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters Atlantis HILIC (150 x 2.1mm,3um,100Å) |
Column Temperature: | 30 |
Flow Gradient: | 0-0.5 min, isocratic 95% B; 0.5-10.5 min, gradient to 40% B; 10.5-15 min, isocratic 40% B; 15-17 min, gradient to 95% B |
Flow Rate: | 250 uL/min |
Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium formate |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | HILIC |
Chromatography ID: | CH003595 |
Chromatography Summary: | HILIC-neg: Hydrophilic interaction liquid chromatography (HILIC) analysis of water soluble metabolites in the negative ionization mode |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Phenomenex Luna NH2 (150 x 2 mm,5um,100Å) |
Column Temperature: | 30 |
Flow Gradient: | 0-10 min, gradient from 90% B to 0% B; 10-12 min, isocratic 0% B; 12-14 min, gradient from 0% B to 90% B |
Flow Rate: | 400 uL/min |
Solvent A: | 100% water; 20 mM ammonium acetate; 20 mM ammonium hydroxide |
Solvent B: | 75% methanol/25% acetonitrile; 10 mM ammonium hydroxide |
Chromatography Type: | HILIC |
Chromatography ID: | CH003596 |
Chromatography Summary: | C8-pos: Reversed-phase C8 chromatography analysis of polar and nonpolar lipids in the positive ionization mode |
Instrument Name: | Shimadzu Nexera X2 |
Column Name: | Waters ACQUITY UPLC BEH C8 (2.1 mm X 100 mm,1.7 µm,130Å) |
Column Temperature: | 40 |
Flow Gradient: | 0-1 min, isocratic 20% B; 1-3 min, gradient to 80% B; 3-10 min, gradient to 100% B; 10-13 min, isocratic 100% B; 13-14 min, gradient to 20% B |
Flow Rate: | 450 uL/min |
Solvent A: | 95% water/5% methanol; 0.1% formic acid; 10 mM ammonium acetate |
Solvent B: | 100% methanol; 0.1% formic acid |
Chromatography Type: | Reversed phase |