Return to study ST002910 main page
MB Sample ID: SA316293
| Local Sample ID: | MDA03-01-4742 |
| Subject ID: | SU003023 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004779 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Bruker Elute |
| Column | Hamilton Intensity Solo 2 C18 (100 mm x 2.1 mm, 1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker timsTOF |
| Ion Mode | POSITIVE |
| Units | AU |
Chromatography:
| Chromatography ID: | CH003611 |
| Chromatography Summary: | For metabolomics analysis, a Hamilton Intensity Solo 2 C18 column (100 mm × 2.1 mm, 1.8 μm beads) was kept at 35 °C. For metabolomics, 10 μL of each sample was injected twice and eluted using a 30-min gradient |
| Instrument Name: | Bruker Elute |
| Column Name: | Hamilton Intensity Solo 2 C18 (100 mm x 2.1 mm, 1.8um) |
| Column Temperature: | 35 |
| Flow Gradient: | a 30-min gradient as follows: 1% ACN was kept for 2 minutes before ramping to 99% ACN for 15 minutes, then holding at 99% ACN for 3 minutes before re-equilibrating to 1% ACN for 10 minutes |
| Flow Rate: | For elution 250 μL/min and 350 μL/min for re-equilibration |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
