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MB Sample ID: SA318246
| Local Sample ID: | fec_s12 |
| Subject ID: | SU003045 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 20 wks |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Lab |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004810 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Synapt-XS Waters |
| Column | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Ion Mobility TOF |
| MS instrument name | Waters Synapt-XS |
| Ion Mode | POSITIVE |
| Units | peak intensities |
Chromatography:
| Chromatography ID: | CH003635 |
| Chromatography Summary: | The LC/MS experiments were conducted using an i-Class LC system coupled to a Synapt-XS mass spectrometer (Waters). Liquid chromatography was performed on fecal, hippocampus, and brainstem samples collected from both the SED and RUN groups. The chromatographic separation was conducted using a BEH HILIC column with 1.7 µm particle size dimensions and 2.1 x 100 mm (Waters). The column temperature was maintained at 50ºC, and the flow rate was set at 0.4 mL/min. The mobile phase consisted of 0.1% formic acid in water as solvent A and 0.1% formic acid in acetonitrile as solvent B. The initial mobile phase composition was 5% A and 95% B, which was held constant for 2 minutes. Subsequently, a linear gradient from 5% to 50% A was applied over 8 minutes, followed by a 2-minute hold at 50% B before returning to the initial condition for a 5-minute re-equilibration. The total run time for each analysis was 19 minutes. |
| Instrument Name: | Synapt-XS Waters |
| Column Name: | Waters ACQUITY UPLC BEH HILIC (100 x 2.1mm,1.7um) |
| Column Temperature: | 50ºC |
| Flow Gradient: | 5% to 50% A was applied over 8 minutes, followed by a 2-minute hold at 50% B before returning to the initial condition for a 5-minute re-equilibration. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
