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MB Sample ID: SA322381
| Local Sample ID: | 20210624_AJU_M1 |
| Subject ID: | SU003074 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004862 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity UHPLC |
| Column | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm, 1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Q Exactive™ Hybrid Quadrupole-Orbitrap MS coupled with a 1290 Infinity UHPLC |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003670 |
| Chromatography Summary: | See protocol file, LC_MS_method_metabolite.pdf |
| Methods Filename: | LC_MS_method_metabolite.pdf |
| Instrument Name: | Agilent 1290 Infinity UHPLC |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (50 x 2.1mm, 1.8um) |
| Column Temperature: | 320℃ |
| Flow Gradient: | 2.5% solvent B in 5 min, 2.5–12.5% solvent B in 29 min, 12.5–25% solvent B in 11 min, 25–37.5% solvent B in 11 min, 37.5-80% solvent B in 0.1 min, holding at 80% of solvent B in 13.9 min, 80–2.5% solvent B in 0.1 min, 2.5% solvent B for 19.9 min |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in 80% acetonitrile |
| Chromatography Type: | Reversed phase |
