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MB Sample ID: SA323086

Local Sample ID:SH_11
Subject ID:SU003086
Subject Type:Other abiotic sample

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Combined analysis:

Analysis ID AN004882
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Orbitrap Exploris 240
Ion Mode POSITIVE
Units raw ion count

Chromatography:

Chromatography ID:CH003684
Chromatography Summary:A published C18 reveres phase method was implemented with minor modifications. The C18 positive method (ESI+) used mobile phase solvents (LC-MS grade) consisting of 0.1% formic acid (Fisher) in water (A) and 0.1% formic acid in methanol (B). The gradient profile was from 0.5% B to 70% B in 4 minutes, from 70% B to 98% B in 0.5 minutes, and holding at 98% B for 0.9 minute before returning to 0.5% B in 0.2 minutes. The flow rate was 350 µL per minute. The sample injection volume was 5 µL. LC separations were made at 40C on separate columns fitted with a Vanguard pre-column of the same composition: Waters Acquity BEH 1.7 µm particle size, 2.1 mm id x 100 mm length (C18). Data were collected at a mass range of 70-1000 m/z at an acquisition rate of 2 spectra per second. Specific ion source parameters included Fragmentor (140V), Gas Temp (250oC), Sheath Gas Temp (200oC), and VCap (4000V).
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um)
Column Temperature:40C
Flow Gradient:From 0.5% B to 70% B in 4 minutes, from 70% B to 98% B in 0.5 minutes, and holding at 98% B for 0.9 minute before returning to 0.5% B in 0.2 minutes.
Flow Rate:0.350 mL/minute
Solvent A:100% water + 0.1% formic acid
Solvent B:100% methanol + 0.1% formic acid
Chromatography Type:Reversed phase
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