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MB Sample ID: SA323095
Local Sample ID: | SH_17 |
Subject ID: | SU003086 |
Subject Type: | Other abiotic sample |
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Combined analysis:
Analysis ID | AN004882 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Orbitrap Exploris 240 |
Ion Mode | POSITIVE |
Units | raw ion count |
Chromatography:
Chromatography ID: | CH003684 |
Chromatography Summary: | A published C18 reveres phase method was implemented with minor modifications. The C18 positive method (ESI+) used mobile phase solvents (LC-MS grade) consisting of 0.1% formic acid (Fisher) in water (A) and 0.1% formic acid in methanol (B). The gradient profile was from 0.5% B to 70% B in 4 minutes, from 70% B to 98% B in 0.5 minutes, and holding at 98% B for 0.9 minute before returning to 0.5% B in 0.2 minutes. The flow rate was 350 µL per minute. The sample injection volume was 5 µL. LC separations were made at 40C on separate columns fitted with a Vanguard pre-column of the same composition: Waters Acquity BEH 1.7 µm particle size, 2.1 mm id x 100 mm length (C18). Data were collected at a mass range of 70-1000 m/z at an acquisition rate of 2 spectra per second. Specific ion source parameters included Fragmentor (140V), Gas Temp (250oC), Sheath Gas Temp (200oC), and VCap (4000V). |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 40C |
Flow Gradient: | From 0.5% B to 70% B in 4 minutes, from 70% B to 98% B in 0.5 minutes, and holding at 98% B for 0.9 minute before returning to 0.5% B in 0.2 minutes. |
Flow Rate: | 0.350 mL/minute |
Solvent A: | 100% water + 0.1% formic acid |
Solvent B: | 100% methanol + 0.1% formic acid |
Chromatography Type: | Reversed phase |