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MB Sample ID: SA325784
Local Sample ID: | K562_100nM_Timecourse_Day6_Porphyrin_1 |
Subject ID: | SU003103 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | K-562 |
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Combined analysis:
Analysis ID | AN004910 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Vanquish |
Column | Phenomenex Kinetex C18 (150 x 3mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Arbitrary Units |
Chromatography:
Chromatography ID: | CH003705 |
Chromatography Summary: | Sample (5 uL) was injected onto a 2.6 μm, 150 x 3 mm C18 column (Phenomenex, 00F-4462-Y0) equipped with a 3.0 mm safe-guard column (Phenomenex, AJ0-8775). Column compartment was heated to 45 ºC. Porphyrins were separated with a chromatographic gradient at a flow rate of 0.800 ml min−1 as follows: 0–2 min: 5% B; 2-19min: linear gradient from 5% to 95% B; 19-21min: 95% B; 21.1-23min: return to 5% B. |
Instrument Name: | Thermo Vanquish |
Column Name: | Phenomenex Kinetex C18 (150 x 3mm,2.6um) |
Column Temperature: | 45 |
Flow Gradient: | linear gradient from 5%B to 95%B |
Flow Rate: | 0.8 mL/min |
Solvent A: | 95% water/5% acetonitrile; 0.1% formic acid |
Solvent B: | 5% water/95% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |