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MB Sample ID: SA326896

Local Sample ID:Cre_24h_C_neg_2
Subject ID:SU003121
Subject Type:Cultured cells
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN004938
Analysis type MS
Chromatography type HILIC
Chromatography system Vanquish Horizon
Column Waters ACQUITY UPLC BEH Amide (100 × 2.1mm, 1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo orbitrap exploris 240
Ion Mode NEGATIVE
Units Peak area

Chromatography:

Chromatography ID:CH003727
Instrument Name:Vanquish Horizon
Column Name:Waters ACQUITY UPLC BEH Amide (100 × 2.1mm, 1.7um)
Column Temperature:25 °C
Flow Gradient:The linear gradient eluted from 95% B (0.0–1 min), 95% B to 65% B (1–7.0 min), 65% B to 40% B (7.0–8.0 min), 40% B (8.0–9.0 min), 40% B to 95% B (9.0–9.1 min), then stayed at 95% B for 5.9 min.
Flow Rate:0.4 mL/min
Solvent A:100% water; 25mM ammonium acetate; 25mM ammonium hydroxider
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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