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MB Sample ID: SA327615
| Local Sample ID: | Sp64 |
| Subject ID: | SU003131 |
| Subject Type: | Mammal |
| Subject Species: | Florida manatee (Trichechus manatus latirostris) |
| Age Or Age Range: | Adult |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004950 | AN004951 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC BEH C18(100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH C18(100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH003735 |
| Chromatography Summary: | The extracted lipid samples, pooled groups, quality controls, and blanks were analyzed by UHPLC-MS/MS on a Thermo Vanquish UHPLC system coupled to a Thermo Q-Exactive Orbitrap mass spectrometer. The column used was a Waters Acquity BEH, 100 mm x 2.1 mm x 1.7 µm. Mobile phase A and B contained 60:40 acetonitrile/water (v/v) and 90:10 isopropanol/acetonitrile (v/v) respectively, with both phases containing 5mM ammonium formate and 0.1% formic acid. The mobile phase gradient was as follows: a linear increase of solvent B from 40% to 55% over 7 min, then increased to 65% by min 8, held at 65% for 4 min, linear increase to 95% over 8 min, increased to 100% over 2 min, solvent B was held at 100% for 5 min before decreasing to 40% in a tenth of a min. Finally, solvent B was held at 100% for another 3 min. The sample injection volume was 10 µL at a flow rate of 0.25 mL/min, the column compartment was 45 °C, and the autosampler temperature was 10 °C. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC BEH C18(100 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Gradient: | a linear increase of solvent B from 40% to 55% over 7 min, then increased to 65% by min 8, held at 65% for 4 min, linear increase to 95% over 8 min, increased to 100% over 2 min, solvent B was held at 100% for 5 min before decreasing to 40% in a tenth of a min. Finally, solvent B was held at 100% for another 3 min. |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 60:40 acetonitrile/water (v/v), 5mM ammonium formate and 0.1% formic acid |
| Solvent B: | 90:10 isopropanol/acetonitrile (v/v), 5mM ammonium formate and 0.1% formic acid |
| Chromatography Type: | Reversed phase |
