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MB Sample ID: SA327977

Local Sample ID:D 43-01-9565
Subject ID:SU003140
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Combined analysis:

Analysis ID AN004961
Analysis type MS
Chromatography type Reversed phase
Chromatography system Bruker Elute
Column Hamilton® Intensity Solo 2 C18 column (2.1 × 100 mm, 1.8 µm)
MS Type ESI
MS instrument type QTOF
MS instrument name Bruker timsTOF
Ion Mode POSITIVE
Units AU

Chromatography:

Chromatography ID:CH003744
Chromatography Summary:The LC-MS/MS analysis utilized an advanced ultra-high-performance liquid chromatography system (UHPLC) provided by Bruker Daltonik GmbH in Bremen, Germany. The analysis employed mobile phases A (water with 0.1% formic acid) and B (acetonitrile with 0.1% formic acid). The gradient program was: 0–2 min, 99% A: 1% B; 2–17 min, 99–1% A: 1–99% B; 17–20 min, 99% B: 1% A. The flow rate was fixed at 0.25 ml/min. Subsequently, 20–20.1 min 99% B to 99% A; 20.1–28.5 min, 99% A: 1% B at 0.35 ml/min flow rate; 28.5–30 min; 99% A: 1% B at 0.25 ml/min. The flow rate was maintained at a constant value throughout the analysis. The sample, in the form of a 10 μl aliquot, was injected into a Hamilton® Intensity Solo 2 C18 column (2.1 mm × 100 mm, 1.8 μm) for separation. The column oven temperature was set to 35 °C
Instrument Name:Bruker Elute
Column Name:Hamilton® Intensity Solo 2 C18 column (2.1 × 100 mm, 1.8 µm)
Column Temperature:35 ◦C
Flow Gradient:The gradient program was: 0–2 min, 99% A: 1% B; 2–17 min, 99–1% A: 1–99% B; 17–20 min, 99% B: 1% A. The flow rate was fixed at 0.25 ml/min. Subsequently, 20–20.1 min 99% B to 99% A; 20.1–28.5 min, 99% A: 1% B at 0.35 ml/min flow rate; 28.5–30 min; 99% A: 1% B at 0.25 ml/min.
Flow Rate:The flow rate was maintained at a constant value throughout the analysis
Solvent A:Water (0.1% Formic Acid)
Solvent B:ACN (0.1% Formic Acid)
Chromatography Type:Reversed phase
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