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MB Sample ID: SA328031
Local Sample ID: | D 65-01-9611 |
Subject ID: | SU003140 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Combined analysis:
Analysis ID | AN004961 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Bruker Elute |
Column | Hamilton® Intensity Solo 2 C18 column (2.1 × 100 mm, 1.8 µm) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Bruker timsTOF |
Ion Mode | POSITIVE |
Units | AU |
Chromatography:
Chromatography ID: | CH003744 |
Chromatography Summary: | The LC-MS/MS analysis utilized an advanced ultra-high-performance liquid chromatography system (UHPLC) provided by Bruker Daltonik GmbH in Bremen, Germany. The analysis employed mobile phases A (water with 0.1% formic acid) and B (acetonitrile with 0.1% formic acid). The gradient program was: 0–2 min, 99% A: 1% B; 2–17 min, 99–1% A: 1–99% B; 17–20 min, 99% B: 1% A. The flow rate was fixed at 0.25 ml/min. Subsequently, 20–20.1 min 99% B to 99% A; 20.1–28.5 min, 99% A: 1% B at 0.35 ml/min flow rate; 28.5–30 min; 99% A: 1% B at 0.25 ml/min. The flow rate was maintained at a constant value throughout the analysis. The sample, in the form of a 10 μl aliquot, was injected into a Hamilton® Intensity Solo 2 C18 column (2.1 mm × 100 mm, 1.8 μm) for separation. The column oven temperature was set to 35 °C |
Instrument Name: | Bruker Elute |
Column Name: | Hamilton® Intensity Solo 2 C18 column (2.1 × 100 mm, 1.8 µm) |
Column Temperature: | 35 ◦C |
Flow Gradient: | The gradient program was: 0–2 min, 99% A: 1% B; 2–17 min, 99–1% A: 1–99% B; 17–20 min, 99% B: 1% A. The flow rate was fixed at 0.25 ml/min. Subsequently, 20–20.1 min 99% B to 99% A; 20.1–28.5 min, 99% A: 1% B at 0.35 ml/min flow rate; 28.5–30 min; 99% A: 1% B at 0.25 ml/min. |
Flow Rate: | The flow rate was maintained at a constant value throughout the analysis |
Solvent A: | Water (0.1% Formic Acid) |
Solvent B: | ACN (0.1% Formic Acid) |
Chromatography Type: | Reversed phase |