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MB Sample ID: SA332250
Local Sample ID: | RF401 |
Subject ID: | SU003187 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005030 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | a.u. |
Chromatography:
Chromatography ID: | CH003801 |
Chromatography Summary: | Analysis of polar metabolites by HILIC chromatography |
Instrument Name: | Thermo Vanquish |
Column Name: | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) |
Column Temperature: | 25 |
Flow Gradient: | 0–20 min: 0–20 min: linear gradient from 20% to 80% B; 20–20.5 min: from 80% to 20% B; 20.5–28 min: hold at 20% B at 150 mL/min flow rate. |
Flow Rate: | 0.25 mL/min |
Solvent A: | 100% acetonitrile |
Solvent B: | 100% water; 20 mM ammonium carbonate, 0.1% ammonium hydroxide |
Chromatography Type: | HILIC |