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MB Sample ID: SA333492
| Local Sample ID: | S25061 |
| Subject ID: | SU003217 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005076 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Ulitmate 3000 |
| Column | SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Arbitrary units |
Chromatography:
| Chromatography ID: | CH003833 |
| Methods Filename: | L12 |
| Chromatography Comments: | The LC column was a MilliporeTM ZIC-pHILIC (2.1 x150 mm, 5 μm) coupled to a Dionex Ultimate 3000TM system and the column oven temperature was set to 25oC for the gradient elution. A flow rate of 100 μL/min was used with the following buffers; A) 10 mM ammonium carbonate in water, pH 9.0, and B) neat acetonitrile. The gradient profile was as follows; 80-20%B (0-30 min), 20-80%B (30-31 min), 80-80%B (31-42 min). Injection volume was set to 2 μL for all analyses (42 min total run time per injection). |
| Instrument Name: | Ulitmate 3000 |
| Column Name: | SeQuant ZIC-pHILIC (150 x 4.6mm,5um) |
| Column Pressure: | 1800 |
| Column Temperature: | 25 |
| Flow Gradient: | 80-20%B (0-30 min), 20-80%B (30-31 min), 80-80%B (31-42 min) |
| Flow Rate: | 0.1mL/min |
| Injection Temperature: | 4 |
| Internal Standard: | ISTD (500nM amino acid cocktail) |
| Sample Injection: | 2uL |
| Solvent A: | 100% water; 10 mM ammonium carbonate, pH 9.0 |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 30m |
| Oven Temperature: | 28 |
| Chromatography Type: | HILIC |
