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MB Sample ID: SA339336
Local Sample ID: | NC-5 |
Subject ID: | SU003251 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005144 |
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Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1260 |
Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | m/z |
Chromatography:
Chromatography ID: | CH003894 |
Chromatography Summary: | Samples were separated on an amide column, using mobile phase A consists of water mixed with 25 mM ammonium acetate and 25 mM Ammonium hydroxide and mobile phase B ACN. The injection volume was 4 µL and flow rate was 0.4 ml/min. 1. The generic HPLC gradient was listed in Table 1: 2. |
Methods Filename: | FUSCC_methods.pdf |
Instrument Name: | Agilent 1260 |
Column Name: | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
Column Temperature: | 350 |
Flow Gradient: | 0.0 min 10% A; 1.0 min 10% A; 11.0 min 13% A; 14.0 min 20% A; 16.5 min 30% A; 18.5 min 50% A; 20.5 min 80% A; 25.0 min 80% A; 25.1 min 10% A; 34.0 min 10% A |
Flow Rate: | 0.4 ml/min |
Solvent A: | 100% water; 25mM ammonium acetate; 25mM ammonium hydroxide |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |