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MB Sample ID: SA340984

Local Sample ID:80MeOH_Blank
Subject ID:SU003269
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:NOD Scid Gamma (NSG)
Age Or Age Range:~4.5 months
Weight Or Weight Range:24.3 +/- 1.9 g (mean +/- SD)
Gender:Female
Animal Housing:Wistar Animal Facility
Species Group:Rodents (order Rodentia)

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Combined analysis:

Analysis ID AN005171 AN005172
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um) Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap Thermo Q Exactive HF-X Orbitrap
Ion Mode NEGATIVE POSITIVE
Units AU AU

Chromatography:

Chromatography ID:CH003912
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um)
Column Temperature:40
Flow Gradient:A linear solvent gradient of a total duration 22.5 min was starting with 0.2 ml/min flow rate of 80% solvent B, 12.5 min – 30%, 15 min – 30%, 15.2 min – 80%, 20 min – 80%, 21 min – flow rate 0.3 ml/min, 22 min – flow rate 0.3 ml/min, 22.1 min – flow rate 0.2 ml/min.
Flow Rate:0.2 ml/min
Solvent A:100% Water; 0.01% ammonium hydroxide; 20 mM ammonium bicarbonate
Solvent B:100% Acetonitrile
Chromatography Type:HILIC
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