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MB Sample ID: SA341050
| Local Sample ID: | STD6_a |
| Subject ID: | SU003271 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005174 | AN005175 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | GC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Agilent 8860 |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) | Agilent J&W DB-35ms (30 m x 0.25 mm, 0.25 µm) |
| MS Type | ESI | EI |
| MS instrument type | Orbitrap | Single quadrupole |
| MS instrument name | Thermo Q Exactive Orbitrap | Agilent 5977C |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Ion Counts (AUC) | Ion Counts (AUC) |
Chromatography:
| Chromatography ID: | CH003914 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 40 |
| Flow Gradient: | The gradient started with 5% of solvent B and 95% solvent A and remained at 5% B until 2 min post injection. A linear gradient to 37% B was carried out until 7 min and increased to 41% until 14 min. Between 14 and 26 minutes the gradient increased to 95% of B and remained at 95% B for 4 minutes. At 30 min the gradient returned to 5% B. The chromatography was stopped at 40 min. |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% water; 10 mM tributyl-amine; 15 mM acetic acid |
| Solvent B: | 100% methanol |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003915 |
| Chromatography Summary: | Inlet Temperature: 270; Helium flow rate: 0.88 mL/min; The GC oven was kept at 100°C and for 1 min, increased with a gradient of 2.5°C min-1 to 105°C for 2 min, then ramped with a gradient of 15°C min-1 to 260°, and after that ramped with a gradient of 20°C min-1 up to 300°C for 5 min. |
| Instrument Name: | Agilent 8860 |
| Column Name: | Agilent J&W DB-35ms (30 m x 0.25 mm, 0.25 µm) |
| Column Temperature: | 100-300 |
| Flow Gradient: | N/A |
| Flow Rate: | N/A |
| Solvent A: | N/A |
| Solvent B: | N/A |
| Chromatography Type: | GC |
