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MB Sample ID: SA342890
| Local Sample ID: | WL_RPLC_POS_WT_3 |
| Subject ID: | SU003281 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005187 | AN005188 | AN005189 | AN005190 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish Flex UHPLC Systems | Thermo Vanquish Flex UHPLC Systems | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | HILICON iHILIC-(P) Classic (100 x 2.1mm,5um) | HILICON iHILIC-(P) Classic (100 x 2.1mm,5um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | QTOF | Orbitrap |
| MS instrument name | Thermo Orbitrap ID-X tribrid | Thermo Orbitrap ID-X tribrid | Agilent 6545 QTOF | Thermo Orbitrap ID-X tribrid |
| Ion Mode | NEGATIVE | NEGATIVE | POSITIVE | POSITIVE |
| Units | peak area | peak area | peak area | peak area |
Chromatography:
| Chromatography ID: | CH003924 |
| Instrument Name: | Thermo Vanquish Flex UHPLC Systems |
| Column Name: | HILICON iHILIC-(P) Classic (100 x 2.1mm,5um) |
| Column Temperature: | 45 |
| Flow Gradient: | 0–1 min: 90% B, 1–12 min: 90-35% B, 12–12.5 min: 35-25% B, 12.5–14.5 min: 25% B |
| Flow Rate: | 250 uL/min |
| Solvent A: | water/acetonitrile (95/5); 20 mM ammonium bicarbonate; 0.1% ammonium hydroxide; 2.5 μM medronic acid |
| Solvent B: | acetonitrile/water (95/5) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003925 |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) |
| Column Temperature: | 60 |
| Flow Gradient: | 0-2min: 30% B, 2-17 min: 30%-75% B, 17-20 min: 75%-85% B, 20-23 min: 85%-100% B, 23-26 min: 100% B |
| Flow Rate: | 250 uL/min |
| Solvent A: | acetronitrile/water (60/40); 10mM ammonium formate; 2.5 uM medronic acid; 0.1% formic acid |
| Solvent B: | 2-propanol/acetonitrile (90/10); 10mM ammonium formate; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
