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MB Sample ID: SA343036

Local Sample ID:D26 19BBz_3
Subject ID:SU003286
Subject Type:Cultured cells
Subject Species:Homo sapiens

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Combined analysis:

Analysis ID AN005196 AN005197
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um) Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um)
MS Type ESI ESI
MS instrument type Exploris120 Exploris120
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE POSITIVE
Units Peak Areas Peak Areas

Chromatography:

Chromatography ID:CH003931
Chromatography Summary:Negative ion Mode
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um)
Column Temperature:45
Flow Gradient:0-0.2 min 5% B, 0.2-.8 min hold at 95% B, .8-.81 min 95-5% B, .81-1 min hold at 5% B.
Flow Rate:0.450 ml/min
Solvent A:100% Water; 10mM Ammonium acetate, .01% Formic Acid
Solvent B:50% Methanol/ 50% Acetonitrile; 10mM Ammonium acetate, .01% Formic Acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH003932
Chromatography Summary:Positive Ion Mode
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Kinetex C18 (30 x 2.1mm, 1.7um)
Column Temperature:45
Flow Gradient:0-0.3 min 5% B, 0.3-.8 min hold at 95% B, .8-.81 min 95-5% B, .81-1 min hold at 5% B.
Flow Rate:0.450 ml/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
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